Zeiss Imager A.1 Fluorescence Microscope

Location: 3118 LSB


The Zeiss Imager A.1 offers the following:

• View with brightfield, darkfield, or phase contrast

• View fluorescence with filters for DAPI, FITC,and Rhodamine.

• 10X, 20X, 40X, or 100X objectives

• Uses a 12 megapixel digital camera to capture images

• Software useful for overlapping images, adding scale bars, measuring length, area, ect. of objects in picture

Filters Include:

1) Differential Interference Contrast (DIC or Nomarski)

2) UV Excitation (for Dapi, Hoechst, Alexa350, AMCA, Marina Blue, APC)

• Excitation BP 377/25, Dichroic FT 415-570

• Emission BP 447/30

3) Blue Excitation (for FITC, GFP, Alexa488, Fluo4)

• Excitation BP 482/40, Dichroic FT 506

• Emission BP 536/25

4) Green Excitation (for Rhodamine, TRITC, DSRed1, Alexa568)

• Excitation BP 546/12, Dichroic FT 580

• Emission LP 590

5) Blue Excitation (for FITC, GFP, Alexa488, Fluo4)

• Excitation BP 450-490, Dichroic FT 510

• Emission LP 515

** Note: If you are not a Life Sciences major then you will not have immediate access to the computer. Please email the technician with your netID at ric_tech@byu.edu and they will have IT add you as a user on the computer.

Additional Information:

• Zeiss Imager A.1 Instructions

Zeiss Imager A.1 Software Instructions

• Zeiss Imager A.1 Software Overlay Instructions

• Zeiss Imager A.1 Immersion Oil Instructions

• Zeiss Imager A.1 Tutorials

More Microscopy info.pdf