Prokaryotes (Bacteria)

Many of our instruments can be used in the study of Prokaryotes. Below are listed some of the uses of our facility in the study of Prokaryotes.

Flow Cytometry

Flow cytometry can be used to study bacterial viability, "gram staining", autofluorescence (GFP and YFP), as well as a few other parameters. Below is an example of a Viability stain.

Bacterial Viability Page

BD FACS Canto Flow Cytometer Page

Microscopy

The Zeiss Axioskop can be used to view stained prokaryotic cells, as well as fluorescence and phase contrast. Below is an example of a phase contrast image of inclusion bodies in bacteria.

Microscopy - Brightfield/Phase Page

Microscopy - Fluorescence Imaging Page

Zeiss Axioskop Page

Imaging

Our Imagers may be used to view an Ethidium Bromide Gel, or some other blot with Prokaryotic DNA, RNA, or Proteins.

Bio-Rad Fluor-S MultiImager Page

Bio-Rad FX Imager Page

Spectrophotometry

Our Nanodrop Spectrophometer can be used to measure concentration and purity of 1 µm samples of DNA or RNA. It can also be used to measure bacterial concentration of samples.

Nanodrop ND-1000 Spectrophometer Page

Spectrophotometry Page

Centrifugation

Our Centrifuges can be used to pellet a bacterial population, and pellet DNA.

Centrifugation - DNA, Proteins, and Viruses Page

Centrifugation - for Cells(Prokaryotes or Eukaryotes) Page

Sorvall Highspeed Centrifuge Page