Using Flow Cytometry one can determine what percentage
of a cell population is in which part of the cell cycle.
The above experiment was done using PI(propidium
which stains DNA and RNA by intercolating between the bases. The
are lyzed using a special procedure which does not disrupt the
which is then stained. The stained nuclei are run through the
and the PI fluorescence of each nucleus is recorded. After we
information we can take the flow data and run it through our
software for cell cycle, Modfit LT 3.0.
Using the Modfit software we can see what
the cell population is in each part of the cell cycle based on
of DNA present.
• G1 phase: 88.78%
• S-phase: 8.58%
• G2/M phase: 2.63%