Using flow cytometry an investigator can determine
percentage of a prokaryotic population is alive and what
is dead. You can also distinguish other forms of viability via
cytometry using a myriad of different viability stains. These
seen with the corresponding paper under additional information
bottom of the page. For the live/dead protocol the population
with two dyes, Syto-9 and PI. Syto-9 stains the DNA of cells
while PI stains only the cells with a compromised membrane.
two stains combined, only live cells are stained with syto-9,
compromised (dead) cells staining with PI.
For this example a 50/50 mix of live and dead
were combined. The cells were stained with the Baclight kit
run on the BD FACSCanto Flow Cytometer. Along the X-Axis is
parameter and the Y-Axis is PI(PE) parameter. We can see from
how close the flow cytometer was at getting the correct
is another example using a 20% live/80% dead mixture.
From these examples we can see the utility of
flow cytometry to study bacterial viability. Please see the
for more information.