Flow Cytometry Software

The RIC facility offers many different ways for investigators to analyze their flow cytometry data. The page will give the basics of the different applications available as well as their differences so one might be better informed of their available options.

BD Biosciences FACSDIVA

This is the software that is used to run the instrument, and which is used by the technician to set up the experiment and acquire samples. Most preliminary analysis is performed using this software. The software is located on the Flow Cytometry computer in 3118 LSB. It is only available on this computer. If you wish to analyze samples outside of the RIC Facility you can use the Summit software.

Cytomation Summit

This software has been made available for the entire university for Flow Cytometric analysis. It has many of the same features as the FACSDIVA software. A copy of the software is available from the lab technician in 3118 LSB. This software is also able to overlay data from different samples onto the same histogram for comparisons. Instructions on how to use the software are located below.

Summit Software Instructions

Summit Software Overlay Instructions

FCS Express

FCS Express

This software is mainly used for publication of data. You can open up your .FCS files into this program and change the way your graphs and histograms look. You can easily add titles and customize your data for publication. Also available with this software is the ability to export your data to a spreadsheet for individual event analysis.

Modfit LT 3.0 DNA analysis

Modfit LT 3.0 DNA analysis

This software is specific to analysis of Flow cytometric DNA samples. It is able to import your .fcs files and give you percentages of G1, S, and G2/M phases. It will also automatically remove debris, aggregates, and give you statistical information of your sample. See the below page for more information.

Cell Cycle

Helpful Links

Flow Cytometer Instructions

Summit Software Instructions

Summit Software Overlay Instructions

Online Fluorescence Spectra tool