Nanodrop ND-1000 Spectrophotometer

Location: 873 WIDB

The NanoDrop® ND-1000 UV-Vis Spectrophotometer enables highly accurate analyses of 1 μl samples with remarkable reproducibility. The patented sample retention system eliminates the need for cuvettes and capillaries which decreases the measurement cycle time. In addition, the high absorbance capability eliminates the need for most dilutions. You’ll find yourself making fewer dilutions and taking more readings than ever before, essentially becoming a more quantitative laboratory.

The ND-1000 offers many benefits:

• Small samples: designed for 1 μl samples

• Large dynamic range: measures 2-3700 ng/μl (dsDNA) on a single sample

• No need to change out cuvettes or caps

• Measures DNA, RNA, and protein concentration

• High absorbance capability: 50 times that of traditional spectrophotometers

• No Dilutions for most samples

• Full Spectrum (220-750nm)

• 10 second measurement time

• No cuvettes or capillaries

Results from a DNA sample

Gives concentration of sample in ng/μl

260/230 ratio = Organic chemicals and solvent contamination

• Important to have this ratio above 1.6 for QPCR

260/280 ratio = Protein Contamination

• Ratios above 1.8 are considered pure

• Sample is recoverable

Additional Information:

Nanodrop Spectrophotometer Instructions

Spectrophotometry